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Real-time PCR Instrument Product List and Ranking from 4 Manufacturers, Suppliers and Companies

Real-time PCR Instrument Product List

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Rotor-Gene Q - Real-time PCR device

Rotor-Gene Q - Real-time PCR device

Real-time PCR system that achieves high sensitivity and high reproducibility.

The Rotor-Gene Q real-time PCR system adopts a revolutionary centrifugal air control method, achieving a temperature variation of ±0.02°C between samples. This high temperature resolution is suitable for experiments such as HRM (High Resolution Melt) analysis and mutation analysis. 【Features】 ■ Achieves high temperature uniformity between samples using centrifugal air control (±0.02°C) ■ No correction reagents are needed due to constant optical path length between samples ■ Ideal for HRM analysis and mutation analysis with high sensitivity and reproducibility ■ No need for regular replacement of heat and light sources *For more details, please feel free to contact us.

  • Real-time PCR

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Real-time PCR device

Real-time PCR device

<Used Analytical Equipment>

This is a multi-wavelength real-time PCR device that uses a copper block with high thermal conductivity. It is equipped with optional filters for HEX/VIC and Cy5. The software includes two types: one for researchers (Thermal Cycler Dice Real Time System) and one for inspectors (for food and environmental testing). (S2872-01)

  • Analytical Equipment and Devices

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Quantitative real-time PCR - 16 wells

Quantitative real-time PCR - 16 wells

It can simultaneously detect 16 samples, and the standard configuration includes two fluorescent channels: FAM/SYBR and VIC.

It can simultaneously detect 16 samples, and the standard configuration includes two fluorescent channels: FAM/SYBR and VIC. It is lightweight and compact, supporting standalone operation. By combining innovative liquid cycle refrigeration technology with Peltier temperature control technology, it achieves rapid heating and cooling. It supports CT value analysis, absolute quantification analysis, isothermal amplification, and endpoint analysis.

  • Analytical Equipment and Devices
  • Other analytical and testing equipment

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[Analysis Case] SNP Analysis Using Real-Time PCR Method

[Analysis Case] SNP Analysis Using Real-Time PCR Method

SNP analysis is possible using real-time PCR (probe method).

Real-time PCR is a method that detects the amplification of DNA fragments from PCR reactions as a fluorescent signal for each cycle. This introduces SNP (Single Nucleotide Polymorphism) analysis using the probe method of real-time PCR. Specific base sequences (probes) labeled with a reporter (fluorescent dye) and a quencher (quenching substance) are used to repeat the three steps shown below: 1) to 3). By using different reporters for each base, the SNP base is identified from the fluorescence intensity. Reactions at each step: 1) Thermal denaturation DNA is converted from double-stranded to single-stranded. 2) Annealing and hybridization Primers and probes specifically bind to single-stranded DNA. 3) Extension reaction The probe is degraded by the extension reaction of the primer, releasing the reporter from the quencher, resulting in fluorescence.

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  • Contract Analysis
  • Contract measurement
  • Contract Inspection

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[Analysis Case] Evaluation of Bacterial Count by Real-Time PCR Method

[Analysis Case] Evaluation of Bacterial Count by Real-Time PCR Method

We will analyze the bacteria in the sample from DNA without isolation or cultivation!

The measurement of bacterial counts conducted in food bacterial testing is calculated by culturing and counting the number of colonies. However, this method requires time for culturing and cannot evaluate difficult-to-culture bacteria. On the other hand, the real-time PCR method allows for the direct extraction of DNA from samples without culturing, enabling the investigation of the quantity of the target bacteria (DNA copy number). This document presents a case study comparing the bacterial counts obtained through culturing the easily cultured Escherichia coli and the copy numbers measured using the real-time PCR method.

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  • Contract measurement
  • Contract Inspection
  • Real-time PCR

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